【Calcein AM Fluorescent Dye】活細胞染劑 Calcein AM 如何配製。TREVIGEN、BIOVISIO 萊恩生物科技有限公司代理 04-25239639。

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活細胞螢光染劑 Calcein AM，用於活細胞觀察及細胞侵入定量實驗, 1755-250

Catalog#: 1755-250 | Size: 5 x 50 µg

活細胞染劑 “Calcein AM”, 用於活細胞觀察及細胞侵入及轉移試劑.

配製方法:

50ug的calcein AM可以溶在50ul的DMSO 最後濃度為1Mm

取1ul的Calcein AM 溶在1X 的1000ul dissociation solution =1uM (1000ul)以96well 為例, 加入100ul的1Um Calcein AM,大約可以使用500次.

Calcein acetoxymethylester (Calcein AM) is a non-fluorescent, cell permeate compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein.

Calcein-AM是一種可對活細胞進行螢光標記的細胞染色試劑，Calcein-AM由於在Calcein(鈣黃綠素)的基礎上加強了疏水性，因此能夠輕易穿透活細胞膜。當其進入到細胞質後，酯酶會將其水解為Calcein(鈣黃綠素)留在細胞內,發出強綠色螢光。

與其他同類試劑（如BCECF-AM和Carboxy-fluorescein diacetate）相比，Calcein-AM是最適合作為螢光探針去染活細胞的，因為它的細胞毒性很低。Calcein(鈣黃綠素)不會抑制任何的細胞功能如增殖或淋巴球的趨化性。另外，使用了Calcein(鈣黃綠素)的活性檢測的實驗結果是十分可信並且與標準的⁵¹Cr-釋放法所得結果相一致的。

Calcein(鈣黃綠素)的激發和發射波長分別為490 nm和515 nm。Calcein-AM和碘化丙啶（PI）溶液，它們分別可對活細胞和死細胞染色。Calcein-AM的乙酸甲基酯親脂性很高，使其可透過細胞膜，通過活細胞內的酯酶作用，Calcein-AM能脫去AM基，產生的Calcein(鈣黃綠素)能發出強綠色螢光，因此Calcein-AM僅對活細胞染色。另一方面，作為核染色染料的PI不能穿過活細胞的細胞膜。它穿過死細胞膜的無序區域而到達細胞核，並嵌入細胞的DNA雙螺旋從而產生紅色螢光（激發：535 nm，發射：617 nm），因此PI僅對死細胞染色。由於Calcein(鈣黃綠素)和PI-DNA都可被490 nm激發，因此可用螢光顯微鏡同時觀察活細胞和死細胞。用545 nm激發，僅可觀察到死細胞。根據以上特點，Calcein-AM和碘化丙啶（PI）經常被結合用來作為活細胞和死細胞的雙重染色。

BIOVISION and TREVIGEN™ Calcein AM Fluorescent Dye can be used to fluorescently label viable cells for a variety of assays such as tumor cell invasion, endothelial cell migration, and endothelial cell tubulogenesis.

Alternate Name/Synonyms: Glycine,N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,bis[(acetyloxy)methyl] ester; Calcein acetoxymethyl ester

Peptide sequence: N/A

Appearance: Off-white solid

Formulation: N/A

CAS Number: 148504-34-1

Molecular Formula: C₄₆H₄₆N₂O₂₃

Molecular Weight: 994.86

Purity: ≥95% by HPLC

Solubility: DMSO (5 mg/ml)

Storage Temp.: -20°C

Shipping Conditions: gel pack

Description: A cell-permeable, non-fluorescent acetoxymethyl ester derivative of Calcein that gets hydrolyzed inside the cell by esterases. The loss of the acetoxymethyl group also enables calcein to readily bind intracellular calcium resulting in a strong yellowish-green fluorescence. It is the best probe for cell viablilty and cell adhesion due to its greater retention in cells as well and its pH insensitivity in physiological range.

Handling: Protect from air and light

USAGE: For Research Use Only! Not For Use in Humans.

更多參考內容:

原廠連結: http://www.trevigen.com/
 

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

4892-010-K Calcein AM螢光染劑使用方法【萊恩生物科技有限公司04-25239639】Trevigen台灣總代理-DNA細胞增殖系列

Calcein AM螢光染劑如何使用【萊恩生物科技有限公司04-25239639】

50ug的calcein AM可以溶在50ul的DMSO 最後濃度為1mM

取1ul的Calcein AM 溶在1X 的1000ul dissociation solution =1uM (1000ul)以96well 為例, 加入100ul的1Um Calcein AM,

大約可以使用500次.

Catalog #	Component	Page Links
4892-010-K	Calcein AM Cell Viability Assay Kit	Cultrex Calcein-AM Cell Viability Assay
6360-025-01	PBR Protein (Recombinant)	PBR
4892-010-02	10X Calcein AM DW Buffer	Cultrex Calcein-AM Cell Viability Assay
4892-010-01	Calcein AM	CultreCoat® 24 Well BME-Coated Cell Invasion Assay Cultrex 24 Well Collagen I Cell Invasion Assay Cultrex 24 Well BME Cell Invasion Assay Cultrex 24 Well Cell Migration Assay Cultrex 24 Well Collagen IV Cell Invasion Assay Cultrex 24 Well Laminin I Cell Invasion Assay Cultrex 96 Well BME Cell Invasion Assay Cultrex 96 Well Cell Migration Assay Cultrex 96 Well Collagen I Cell Invasion Assay Cultrex 96 Well Collagen IV Cell Invasion Assay Cultrex 96 Well Endothelial Cell Invasion Kit Cultrex 96 Well Laminin I Cell Invasion Assay Cultrex Calcein-AM Cell Viability Assay Cultrex In Vitro Angiogenesis Assay - Tube Formation Kit

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

WST螢光分析細胞增殖 (Cell proliferation and viability)Biovision代理【萊恩生物科技有限公司】

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

螢光細胞增生分析套組, BIOVISION K303-500
VisionBlue™ Quick Cell Viability Assay
Catalog#: K303-500 | Size: 500 assays
Click to enlarge 安全性佳, 除了測量細胞增殖分析外, 可以再進行下游分析
Very safe: Non-radioactive, non-toxic, and cells can be further used for other experiments..
Kit Summary:
• Detection method- Fluorescence (Ex/Em 530–570/ 590-620 nm) • Sample type- Cell culture (adherent and suspension) • Species reactivity- Mammalian • Kit size- Convenient sizes (500, 2500) • Applications- This fluorescence-based single-step cell viabilty assay utilizes the redox dye (Resazurin) which is not fluorescent, but upon reduction by metabolically active cells, it becomes highly fluorescent (Ex = 530-570 nm; Em = 590 -620 nm). Therefore, viable metabolically active cells can be easily measured.
Features & Benefits:
• Simple one-step procedure • Fast and convenient • The assay is more sensitive than other similar assays (eg, Almar Blue reagent) on the market
Kit components:
• VisionBlue™ Reagent
Description:
The VisionBlue™ Quick Cell Proliferation Assay Kit provides by far the most sensitive and easiest means for quantifying cell proliferation and viability. Simply add the single reagent to cell culture, incubate and read the fluorescence intensity. The VisionBlue™assay utilizes the redox dye resazurin which is not fluorescent, but upon reduction by viable metabolically active cells, the dye becomes highly fluorescent (Ex = 530-570 nm; Em = 590-620 nm). Therefore, viable metabolically active cells can be easily measured.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans


.

The Quick Cell Proliferation Assay Kit provides all reagents and detailed instructions for a fast and sensitive quantification of cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells resulted in an increase in the activity of the mitochondrial dehydrogenases, which leads to the increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, requiring no washing, no harvesting, and no solubilization steps, and is faster and more sensitive than MTT, XTT, or MTSbased assays. The entire assay can be performed in a microtiter plate.

Catalog #	Product Name+	Size
K301-2500	Quick Cell Proliferation Assay Kit	2500 assays
K301-500	Quick Cell Proliferation Assay Kit	500 assays
K302-2500	Quick Cell Proliferation Assay Kit Plus	2500 assays
K302-500	Quick Cell Proliferation Assay Kit Plus	500 assays
K303-2500	VisionBlue™ Quick Cell Viability Assay	2500 assays
K303-500	VisionBlue™ Quick Cell Viability Assay	500 assays

BIOVISION粒線體染劑JC-1 (Catalog#1130-5)，測量粒線體膜電位改變【萊恩生物科技有限公司04-25239639】BIOVISION代理

Biovision www.biovision.com

JC-1 ( 5, 5’ ,6, 6’ -tetrachloro-1, 1’ ,3, 3’ -tetraethyl-benzimidazolylcarbocyanine Iodide_Catalog#: 1130-5 | Size: 5 mg

JC-1 染劑是一種粒線體染劑(mitochondrial dye)。JC-1染劑可以測量粒線體膜電位，染出粒線體膜電位失衡現象。其原理為: 當帶正電的JC-1染劑進入細胞中，會被粒線體膜上的負性電場所吸引並聚集形成二聚體，在螢光顯微鏡下發出橘組色螢光；若是粒線體上膜電位消失，使JC-1染劑無法被膜上電場所吸引，而散佈在細胞中，並呈現單體狀態，在螢光顯微鏡下發出綠色螢光。JC-1染劑可以用來觀察細胞凋亡現象，在健康的細胞中，發出紅色螢光；當細胞胞凋亡發生時，發出綠色染光。

Description: JC-1 is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 monomer is in equilibrium with so called J-aggregates, which are favored at higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (lem = 527 nm), while the J-aggregates have red fluorescence (lem = 590 nm). Therefore, it has been possible to use fluorescence ratioing technique to study mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy cells, the dye aggregates in the mitochondria and fluoresces red.

詳細產品資訊，請登錄www.bivision.com 查詢
 

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

 

SouthernBiotech [DNA活性染劑7-AAD] 7-Aminonactinomycin D(Catalog#1130-5)【萊恩生物科技有限公司】

7-Aminoactinomycin D (7-AAD) DNA活性染劑

美國SouthernBiotech公司

貨號: 10042-01

品名: 7-Aminoantinomycin D (7-AAD)

規格: 100 tests

7-AAD （7-amino-actinomycin D）是一種活性核酸染料，它不能通過正常細胞質，隨著細胞凋亡、細胞死亡過程，細胞質對7-AAD的通透性逐漸增加，進而結合細胞凋亡中的DNA，在合適波長激發光的激發下可發出明亮的紅色螢光。經由7-AAD標記DNA的強弱，可將細胞分為三種：7-AAD染劑表現強螢光時，代表為死亡細胞，7-AAD弱是凋亡細胞，7-AAD-為正常活力細胞。7-AAD與PI 一樣有著相似的螢光特性，但其發射波譜較PI窄，對其他檢測通道的幹擾更小，在多色螢光分析中是PI 的最佳替代品，可與Annexin V-EGFP/FITC/PE聯合使用。

7-AAD適用於培養的細胞染色，可應用於螢光顯微鏡、鐳射共聚焦儀器或流式細胞儀檢測。

螢光顯微鏡觀察

1． 滴一滴上述染色後的細胞懸液於載玻片上，並用蓋玻片蓋上細胞；

2．對於貼壁細胞來說，也可直接用蓋玻片來培養細胞並誘導細胞凋亡；

（1）將細胞于蓋玻片上生長，用適當的凋亡誘導劑誘導細胞凋亡，並設立陰性對照組；

（2）用PBS洗滌細胞兩次；

（3）在500μL 的1×Assays Buffer中加入5μL7-AAD染液，混勻；

（4）將上述溶液滴加于蓋玻片表面，使長有細胞的蓋玻片表面均勻覆蓋；

（5）避光、室溫反應5 min。

3． 將蓋玻片倒置於載玻片上，於螢光顯微鏡下觀察激發波長546nm，發射波長647 nm，7-AAD螢光信號呈紅色。

Flow cytometry analysis of viable cells. Product can be used in conjunction with R-PE (R-phycoerythrin) and FITC (fluorescein) antibodies in 2-color analysis due to minimal emissions overlap. Product can also be used as a vital dye with the following SouthernBiotech Annexin V conjugates:

10040-09 Annexin V-R-PE

10040-11 Annexin V-APC

10040-15 Annexin V-CY?5

詳細產品資訊，請登錄www.southernbiotech.com 查詢，或與萊恩生物科技有限公司(04)25239639聯繫。

更多參考內容:

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

TREVIGEN細胞凋亡Apoptosis分析。塑化風波燒出食品安全，天然的尚好。TREVIGEN提供全面細胞凋亡研究分析。【萊恩生物科技有限公司】Biovison.Trevigen台灣總代理

塑化風波燒出食品安全，天然的尚好。

BIOVISON.TREVIGEN提供全面【DnBP、DEHP、塑化劑】對細胞凋亡Apoptosis研究、分析。【太鼎生物科技有限公司02-86609496】Biovison.Trevigen台灣總代理

Apoptosis
Cell Membrane Events

• Annexin V Kits
• TACS^® Annexin V-FITC Kit
• TACS^® Annexin V-Biotin Kit

Cell Proliferation

• TACS^® MTT Cell Proliferation Assay
• TACS^® XTT Cell Proliferation Assay

Mitochondria-Related Events

• DePsipher^TM Mitochondrial Potential Assay
• MitoShift^TM Mitochondrial Potential Assay
• Antibodies to Mitochondria-Related Proteins
  • Bax Monoclonal Antibodies
  • Bcl-2 Monoclonal Antibodies
  • Bcl-XL Monoclonal Antibodies
  • PBR Polyclonal Antibody

Apoptosis-Related Antibodies

• Cleaved Caspase 3 Polyclonal Antibody
• G3PDH Polyclonal Antibody

DNA Fragmentation

• TACS^® In Situ Apoptosis Detection Kits (TUNEL Assays)
  • TACS·XL^® Kits
  • TACS^® 2 TdT Kits

Specialty In Situ Apoptosis Detection Kits (TUNEL Assays)

• CardioTACS^™ Kit
• DermaTACS^™ Kit
• NeuroTACS^™ II Kit
• TumorTACS^™ Kit
• TiterTACS^™ Kit
• FlowTACS^™ Kit
• VasoTACS^™ Kit

DNA Laddering Kits

• Ethidium Bromide DNA Laddering Kit
• Tissue Extraction Reagents

Service and Accessories

• Apoptosis Inducers
• Apoptosis Grade^™ PBS Buffer
• Apoptosis Grade^™ Water
• Control Slides
• Counterstains
• Mounting Media
• Permeabilization Reagents
• Slides and Coverslips
• Streptavidin Conjugates

詳細產品資訊，請登錄www.trevigen.com 查詢
 

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

【細胞活性冷光分析] ApoSENSOR™ ATP Cell Viability Assay Kit ATP冷光細胞活性分析套組BIOVISION台灣代理經銷商Catalog#: K254-200

ApoSENSOR™ ATP Cell Viability Assay Kit 【細胞活性ATP分析系列產品】 ATP冷光細胞活性分析套組 【供應廠商】萊恩生物科技有限公司 【公司電話】04-2523-9639 【業務專員】陳俊豪 0937-229803 【電子信箱】biolion0819@gmail.com Catalog#: K254-200 | Size: 200 assays

Product Details Kit Summary: • Detection method- Luminometer or Beta Counter. • Sample type- Cell and Tissue lysates, culture media, urine, soil, sludge, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Kit size- 200, 1000 assays • Applications- Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells. Features & Benefits: • Simple one-step procedure; takes only 30 minutes • Fast and convenient • The assay can be done directly in culture plates requiring no harvest/washing/or sample preparations. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well). Kit components: • Nucleotide Releasing Buffer • ATP Monitoring Enzyme • Enzyme Reconstitution Buffer • ATP (MW 551) Description: Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in cell has been used as an indicator of cell death. In contrast, cell proliferation has been recognized by increased levels of ATP. The ApoSENSOR™ Cell Viability Assay Kit utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is extremely sensitive (detects 10-100 mammalian cells/well). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge. Storage Conditions: -70°C Shipping Conditions: gel pack USAGE: For Research Use Only! Not For Use in Humans. 其它相關產品 7902-100 StayBrite™ D-Luciferin, Sodium Salt 100 mg 7902 -1G StayBrite™ D-Luciferin, Sodium Salt 1 g 7902 -10G StayBrite™ D-Luciferin, Sodium Salt 10 g K791-100 StayBrite™ Highly Stable ATP Assay Kit 100 assays K791-1000 StayBrite™ Highly Stable ATP Assay Kit 1000 assays 7901-150 StayBrite™ Highly Stable Luciferase 150 ug K790-100 StayBrite™ Highly Stable Luciferase/Luciferin Reagent 100 assays K790-1000 StayBrite™ Highly Stable Luciferase/Luciferin Reagent 1000 assays K790-10000 StayBrite™ Highly Stable Luciferase/Luciferin Reagent 10000 assays