Annexin V螢光凋亡分析試劑組 另有多種螢光分析試劑 Annexin-FITC, Annexin-Cy3, Annexin-EGFP, Annexin-Cy5, Annexin-PE, Annexin-Biotin

萊恩生物科技公司提供多種Annexin V凋亡分析套組

Flow Cytometry流式細胞儀專用螢光分析試劑

BioVision公司提供高品質的膜蛋白Annexin V 產品的快速檢測活細胞的凋亡。 Annexin V的結合物是專門設計生產增強螢光訊號和耐光性，大大優於其他同類產品在市場上的表現。 Annexin V的檢測細胞凋亡就是這麼簡單，只需要10分鐘。結果可以通過流式細胞儀或螢光顯微鏡進行分析!!

Annexin V BioVision offers high quality annexin V products for fast detection of apoptosis in living cells. Annexin V conjugates are specially engineered to produce enhanced fluorescence signals and photostability, which perform considerably better than other similar products in the market. Annexin V Apoptosis Assay is so simple, takes only 10 minutes. Results can be analyzed by flow cytometry or fluorescence microscopy. Complete kits of 25, 100, 400 assays sizes are available. Bulk annexin V reagents of 1000 assays size are also available, separately.

Annexin V Binding Buffer Annexin V, Unlabelled Annexin V-Biotin Annexin V-Cy3 Annexin V-Cy5 Annexin V-EGFP Annexin V-FITC Annexin V-PE Annexin V-PE-Cy5 Propidium Iodide

K101-100	Annexin V-FITC Apoptosis Kit	100 assays
K101-25	Annexin V-FITC Apoptosis Kit	25 assays
K101-400	Annexin V-FITC Apoptosis Kit	400 assays
K201-100	Annexin V-FITC Apoptosis Kit Plus	100 assays
K201-25	Annexin V-FITC Apoptosis Kit Plus	25 assays
K201-400	Annexin V-FITC Apoptosis Kit Plus	400 assays
1001-1000	Annexin V-FITC Reagent	1000 assays
1001-200	Annexin V-FITC Reagent	200 assays

1056-1

Propidium Iodide

1 ml

供應廠商   萊恩生物科技有限公司

連絡電話   04-25239639

聯絡專員   陳俊豪 0937-229803

電子信箱    biolion0819@gamil.com

BioVision Apoptosis Inhibitors凋亡抑制劑 Caspase & Granzyme B Inhibitors, 還有相關Apoptosis Inducers

提供Caspase & Granzyme B Inhibitors相關抑制劑, 還有相關誘導製劑!!

1124-20C	Biotin-DEVD-FMK	20 µl (10 mM)
1121-20C	Biotin-IETD-FMK	20 µl (10 mM)
1123-20C	Biotin-VAD-FMK	20 µl (10 mM)
1985-500	Biotinyl-ASTD-FMK	500 µg
1985-1000	Biotinyl-ASTD-FMK	1 mg
1160-1	Boc-D-FMK	1 mg
1160-5	Boc-D-FMK	5 mg
1120-20C	Caspase Inhibitor Boc-D-FMK	20 µl (10 mM)
1012-100	Caspase-1 Inhibitor Z-YVAD-FMK	100 µl
1012-20C	Caspase-1 Inhibitor Z-YVAD-FMK	20 µl
1112-100	Caspase-10 Inhibitor AEVD-FMK	100 µl (2 mM)
1112-20C	Caspase-10 Inhibitor AEVD-FMK	20 µl (10 mM)
1079-100	Caspase-12 Inhibitor Z-ATAD-FMK	100 µl (2 mM)
1079-20C	Caspase-12 Inhibitor Z-ATAD-FMK	20 µl (10 mM)
1115-100	Caspase-13 Inhibitor LEED-FMK	100 µl (2 mM)
1115-20C	Caspase-13 Inhibitor LEED-FMK	20 µl (10 mM)
1073-100	Caspase-2 Inhibitor Z-VDVAD-FMK	100 µl (2 mM)
1073-20C	Caspase-2 Inhibitor Z-VDVAD-FMK	20 µl (10 mM)
1009-100	Caspase-3 Inhibitor Z-DEVD-FMK	100 µl
1009-20C	Caspase-3 Inhibitor Z-DEVD-FMK	20 µl
1108-100	Caspase-4 Inhibitor Z-LEVD-FMK	100 µl (2 mM)
1108-20C	Caspase-4 Inhibitor Z-LEVD-FMK	20 µl (10 mM)
1100-100	Caspase-5 Inhibitor Z-WEHD-FMK	100 µl (2 mM)
1100-20C	Caspase-5 Inhibitor Z-WEHD-FMK	20 µl (10 mM)
1011-100	Caspase-6 Inhibitor Z-VEID-FMK	100 µl
1011-20C	Caspase-6 Inhibitor Z-VEID-FMK	20 µl
1064-100	Caspase-8 Inhibitor Z-IETD-FMK	100 µl (2 mM)
1064-20C	Caspase-8 Inhibitor Z-IETD-FMK	20 µl (10 mM)
1074-100	Caspase-9 Inhibitor Z-LEHD-FMK	100 µl (2 mM)
1074-20C	Caspase-9 Inhibitor Z-LEHD-FMK	20 µl (10 mM)
K107-5-25	Caspase-Family Inhibitor Set	5 x 25 µl
K108-7-25	Caspase-Family Inhibitor Set II	7 x 25 µl
K210-8-25	Caspase-Family Inhibitor Set III	8 x 25 µl
K220-12-25	Caspase-Family Inhibitor Set IV	12 x 25 µl
1010-100	Caspase-Family Inhibitor Z-VAD-FMK	100 µl
1010-20C	Caspase-Family Inhibitor Z-VAD-FMK	20 µl
1127-20C	EMAP-II Inhibitor Z-ASTD-FMK	20 µl (10 mM)
1173-1	EZSolution™ Q-VD-OPh	1 mg
1174-1	EZSolution™ Q-VD-OPh, Negative Control	1 mg
1122-100	FMK Negative Control	100 µl (2 mM)
1122-20C	FMK Negative Control	20 µl (10 mM)
1119-1	Granzyme B Inhibitor Ac-IEPD-CHO	1 mg
1128-20C	Granzyme B Inhibitor Z-AAD-CH2Cl	20 µl (10 mM)
1170-3	Q-VD-OPh	3 mg
1170-1	Q-VD-OPh	1 mg
1170-5	Q-VD-OPh	5 mg
1171-1	QVD-OPh Negative Control	1 mg
1171-5	QVD-OPh Negative Control	5 mg
1171-3	QVD-OPh Negative Control	3 mg
1152-1	Z-ATAD-FMK	1 mg
1152-5	Z-ATAD-FMK	5 mg
1143-1	Z-DEVD-FMK	1 mg
1143-5	Z-DEVD-FMK	5 mg
1148-1	Z-IETD-FMK	1 mg
1148-5	Z-IETD-FMK	5 mg
1149-1	Z-LEHD-FMK	1 mg
1149-5	Z-LEHD-FMK	5 mg
1144-1	Z-LEVD-FMK	1 mg
1144-5	Z-LEVD-FMK	5 mg
1140-1	Z-VAD-FMK	1 mg
1140-5	Z-VAD-FMK	5 mg
1142-1	Z-VDVAD-FMK	1 mg
1142-5	Z-VDVAD-FMK	5 mg
1146-1	Z-VEID-FMK	1 mg
1146-5	Z-VEID-FMK	5 mg
1141-1	Z-YVAD-FMK	1 mg

Apoptosis Inducers            相關誘導劑

1036-50	Actinomycin D (10 mM)	50 µl
1549-10	Anisomycin	10 mg
2380-5	AT-101	5 mg
2380-25	AT-101	25 mg
1830-100	Berberine Chloride	100 mg
1830-500	Berberine Chloride	500 mg
1552-25	Betulinic acid	25 mg
1560-5	Brefeldin A	5 mg
2390-5	C2 Ceramide	5 mg
2390-25	C2 Ceramide	25 mg
1579-5	Cerulenin	5 mg
1579-50	Cerulenin	50 mg
1519-1000	Colchicine	1 g
1041-1G	Cycloheximide	1 g
1041-1	Cycloheximide (100 mM)	1 ml
1524-10	Daunorubicin.HCl	10 mg
1042-10G	Dexamethasone	10 g
1042-1G	Dexamethasone	1 g
1527-5	Doxorubicin.HCl	5 mg
1043-250MG	Etoposide	250 mg
1043-25MG	Etoposide	25 mg
1043-100	Etoposide (100 mM)	100 µl
2297-5	LY573636	5 mg
2297-25	LY573636	25 mg
2426-50	Phytosphingosine	50 mg
2426-250	Phytosphingosine	250 mg
1551-1000	Tamoxifen citrate	1 g

公        司   萊恩生物科技有限公司

電        話   04-25239639

聯  絡  人   陳俊豪  0937-229803

電子信箱   biolion0819@gmail.com

Biovision 提供多種 Apoptosis Inducers 凋亡誘導藥物

新產品 LY573636
一個強有力的抗腫瘤劑，可導致細胞生長停滯和凋亡誘導體外和體內的腫瘤。 LY573636誘導細胞凋亡發生，藉由線粒體的引導機制。
Description: A potent anti-tumor agent, which causes growth arrest and apoptosis of a variety of human solid tumors in vitro and in vivo. LY573636-induced apoptosis occurs by a mitochondrial-targeted mechanism.

網頁連結 http://www.biovision.com/biochemicals-741/apoptosis-inducers-745/

1036-50	Actinomycin D (10 mM)	50 µl	$65.00
1549-10	Anisomycin	10 mg	$95.00
1830-100	Berberine Chloride	100 mg	$55.00
1830-500	Berberine Chloride	500 mg	$195.00
1552-25	Betulinic acid	25 mg	$79.00
1560-5	Brefeldin A	5 mg	$85.00
1579-5	Cerulenin	5 mg	$110.00
1579-50	Cerulenin	50 mg	$725.00
1519-1000	Colchicine	1 g	$88.00
1041-1G	Cycloheximide	1 g	$95.00
1041-1	Cycloheximide (100 mM)	1 ml	$70.00
1524-10	Daunorubicin.HCl	10 mg	$75.00
1042-10G	Dexamethasone	10 g	$495.00
1042-1G	Dexamethasone	1 g	$99.00
1527-5	Doxorubicin.HCl	5 mg	$75.00
1043-250MG	Etoposide	250 mg	$395.00
1043-25MG	Etoposide	25 mg	$79.00
1043-100	Etoposide (100 mM)	100 µl	$65.00
2297-5	LY573636	5 mg	$75.00
2297-25	LY573636	25 mg	$295.00
1551-1000	Tamoxifen citrate	1 g	$99.0

萊恩生物科技公司

台中 04-25239639

業務專員 陳俊豪 0937-229803

電子信箱  biolion0819@gmail.com

MTT細胞增生分析套組

提供多種細胞增生分析試劑

MTT：3- (4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
MTT為黃色化合物，是一種接受氫離子的染料，可作用於活細胞線粒體中的呼吸鏈，在琥珀酸脫氫酶(SDH)和細胞色素C的作用下tetrazolium環開裂，生成藍色的formazan結晶，formazan結晶的生成量與活細胞數目成正比（死細胞中琥珀酸脫氫酶消失，不能將MTT還原）。
可利用測O.D.值得知細胞還原MTT的能力(formazan形成量)，此O.D.值代表了粒線體的活性，即活細胞數目，故MTT assay可用作細胞存活率的指標。

Catalog #	Product Name	Size
4890-25-01	MTT Reagent	25 ml
4890-25-02	MTT Detergent Reagent	250 ml
4890-025-K	MTT Cell Proliferation Assay Kit	2500 Wells
4890-050-K	MTT Cell Proliferation Assay Kit	5000 Wells

另有多種生化試劑提供研究人員,用於細胞程式性死亡,DNA損傷,凋亡和修復等研究

對於腫瘤研究提供多種高品質的試劑組,開發一連串相關試劑如血管生成分析套組,
細胞侵襲腫瘤形成幹細胞研究,氧化壓力損傷,等相關分析試劑的提供...

DNA Damage & Genomic Instability

DNA Repair Gene Knockdown Cell Lines

PARP/PAR/PARG

CometAssay®

DNA Damage Antibodies

DNA Repair Enzymes

Cancer Cell Behavior

Cultrex® Basement Membrane Extract (BME)

Cultrex® ECM Component Proteins and Reagents

Cancer Cell Behavior Assays

3-D Products

Apoptosis Detection

Annexin V Assays

DNA Fragmentation

Mitochondrial Events

Cell Proliferation

Antibodies

Apoptosis Research Accessories

Stem Cell Products

Stem Cell Qualified Basement Membrane Extract Matrix

Rat Mesenchymal Stem Cell Growth and Replenishment products

Stem Cell Qualified ECM Proteins

Oxidative Stress

HT 8-oxo-dG ELISA KIT I & II

Superoxide Dismutase Assay Kits (SOD)

Glutathione Assay Kits

8-oxo-dG Monoclonal Antibody (clone 2E2)

 

Molecular Biology Reagents

Genomic DNA Isolation Kit

Orange G Loading Dye

Electrophoresis Buffer

PBS

TdT DNA Polymerase

Calf Thymus DNA, Herring Sperm DNA,Salmon Sperm DNA

Proteinase K

 

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

                        

【Calcein AM Fluorescent Dye】活細胞染劑 Calcein AM 如何配製。TREVIGEN、BIOVISIO 萊恩生物科技有限公司代理 04-25239639。

• 分享在我的 Facebook
• 分享在我的 Plurk
• 分享在我的即時通

活細胞螢光染劑 Calcein AM，用於活細胞觀察及細胞侵入定量實驗, 1755-250

Catalog#: 1755-250 | Size: 5 x 50 µg

活細胞染劑 “Calcein AM”, 用於活細胞觀察及細胞侵入及轉移試劑.

配製方法:

50ug的calcein AM可以溶在50ul的DMSO 最後濃度為1Mm

取1ul的Calcein AM 溶在1X 的1000ul dissociation solution =1uM (1000ul)以96well 為例, 加入100ul的1Um Calcein AM,大約可以使用500次.

Calcein acetoxymethylester (Calcein AM) is a non-fluorescent, cell permeate compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein.

Calcein-AM是一種可對活細胞進行螢光標記的細胞染色試劑，Calcein-AM由於在Calcein(鈣黃綠素)的基礎上加強了疏水性，因此能夠輕易穿透活細胞膜。當其進入到細胞質後，酯酶會將其水解為Calcein(鈣黃綠素)留在細胞內,發出強綠色螢光。

與其他同類試劑（如BCECF-AM和Carboxy-fluorescein diacetate）相比，Calcein-AM是最適合作為螢光探針去染活細胞的，因為它的細胞毒性很低。Calcein(鈣黃綠素)不會抑制任何的細胞功能如增殖或淋巴球的趨化性。另外，使用了Calcein(鈣黃綠素)的活性檢測的實驗結果是十分可信並且與標準的⁵¹Cr-釋放法所得結果相一致的。

Calcein(鈣黃綠素)的激發和發射波長分別為490 nm和515 nm。Calcein-AM和碘化丙啶（PI）溶液，它們分別可對活細胞和死細胞染色。Calcein-AM的乙酸甲基酯親脂性很高，使其可透過細胞膜，通過活細胞內的酯酶作用，Calcein-AM能脫去AM基，產生的Calcein(鈣黃綠素)能發出強綠色螢光，因此Calcein-AM僅對活細胞染色。另一方面，作為核染色染料的PI不能穿過活細胞的細胞膜。它穿過死細胞膜的無序區域而到達細胞核，並嵌入細胞的DNA雙螺旋從而產生紅色螢光（激發：535 nm，發射：617 nm），因此PI僅對死細胞染色。由於Calcein(鈣黃綠素)和PI-DNA都可被490 nm激發，因此可用螢光顯微鏡同時觀察活細胞和死細胞。用545 nm激發，僅可觀察到死細胞。根據以上特點，Calcein-AM和碘化丙啶（PI）經常被結合用來作為活細胞和死細胞的雙重染色。

BIOVISION and TREVIGEN™ Calcein AM Fluorescent Dye can be used to fluorescently label viable cells for a variety of assays such as tumor cell invasion, endothelial cell migration, and endothelial cell tubulogenesis.

Alternate Name/Synonyms: Glycine,N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,bis[(acetyloxy)methyl] ester; Calcein acetoxymethyl ester

Peptide sequence: N/A

Appearance: Off-white solid

Formulation: N/A

CAS Number: 148504-34-1

Molecular Formula: C₄₆H₄₆N₂O₂₃

Molecular Weight: 994.86

Purity: ≥95% by HPLC

Solubility: DMSO (5 mg/ml)

Storage Temp.: -20°C

Shipping Conditions: gel pack

Description: A cell-permeable, non-fluorescent acetoxymethyl ester derivative of Calcein that gets hydrolyzed inside the cell by esterases. The loss of the acetoxymethyl group also enables calcein to readily bind intracellular calcium resulting in a strong yellowish-green fluorescence. It is the best probe for cell viablilty and cell adhesion due to its greater retention in cells as well and its pH insensitivity in physiological range.

Handling: Protect from air and light

USAGE: For Research Use Only! Not For Use in Humans.

更多參考內容:

原廠連結: http://www.trevigen.com/
 

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

4892-010-K Calcein AM螢光染劑使用方法【萊恩生物科技有限公司04-25239639】Trevigen台灣總代理-DNA細胞增殖系列

Calcein AM螢光染劑如何使用【萊恩生物科技有限公司04-25239639】

50ug的calcein AM可以溶在50ul的DMSO 最後濃度為1mM

取1ul的Calcein AM 溶在1X 的1000ul dissociation solution =1uM (1000ul)以96well 為例, 加入100ul的1Um Calcein AM,

大約可以使用500次.

Catalog #	Component	Page Links
4892-010-K	Calcein AM Cell Viability Assay Kit	Cultrex Calcein-AM Cell Viability Assay
6360-025-01	PBR Protein (Recombinant)	PBR
4892-010-02	10X Calcein AM DW Buffer	Cultrex Calcein-AM Cell Viability Assay
4892-010-01	Calcein AM	CultreCoat® 24 Well BME-Coated Cell Invasion Assay Cultrex 24 Well Collagen I Cell Invasion Assay Cultrex 24 Well BME Cell Invasion Assay Cultrex 24 Well Cell Migration Assay Cultrex 24 Well Collagen IV Cell Invasion Assay Cultrex 24 Well Laminin I Cell Invasion Assay Cultrex 96 Well BME Cell Invasion Assay Cultrex 96 Well Cell Migration Assay Cultrex 96 Well Collagen I Cell Invasion Assay Cultrex 96 Well Collagen IV Cell Invasion Assay Cultrex 96 Well Endothelial Cell Invasion Kit Cultrex 96 Well Laminin I Cell Invasion Assay Cultrex Calcein-AM Cell Viability Assay Cultrex In Vitro Angiogenesis Assay - Tube Formation Kit

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com