2012年12月10日 星期一

ATP冷光儀 KIKKOMAN-PD20 攜帶型 針對食品工廠 快速檢測 醫療器具 環境汙染指標測定

【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

 
Kikkoman Hygiene
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Surface Hygiene Monitoring ATP System

kikkomanWherever food residue is present, there is a risk of microbial contamination. While culture tests can detect the presence of microorganisms, ATP tests also detect the presence of food residues and therefore provides a more complete indicator of risk. Also, culture tests take several days for a complete measurement while ATP results are available within seconds.
The Kikkoman LuciPac ATP pen is designed specifically for measuring contamination on surfaces in the food & medical hygiene industries. When used together with the Kikkoman Lumitester PD-20 luminometer, surface contamination can be detected anywhere at any time. LuciPac tests are best suited for cleanliness control in food manufacturing and processing plants, transportation and distribution facilities, supermarkets, and food preparation facilities such as kitchens. Medical applications such as hospitals and clinics can also benefit from LuciPac technology.
Advantages of Kikkoman surface tests:
  • Small and lightweight – The PD-20 is the world's smallest luminometer (235 g, without battery).
  • Simple and speedy – "Swab and measure. That's all". Total measuring time is only 10 seconds.
  • High sensitivity – in addition to measuring ATP, LuciPac tests also measure AMP (Adenosine Monophosphate), a molecule generated from ATP during processing, such as heating and fermentation.
Note: LuciPac pens and PD-20 luminometer are not compatible for use in fluid applications, nor are they interchangable with other LuminUltra products.



日本Kikkoman公司生產的手持式環境ATP檢測冷光儀, 有別於傳統平板計數法, 此機型可以在現場直接完成偵測, 且在10秒內即可得到檢測結果!!
產品介紹:
1) PD-20為掌上型環境衛生檢測儀,大小就像一般倉儲所使用的條碼機,配合拋棄式樣品採檢棒,對於廠商內部衛生管理人員或是衛生單位來說,不但可以隨身攜帶至食品製造各個檢查點,即時進行現場檢測,並可在十秒內以儀器讀值確認食品製造或處理過程中所使用到之容器或用具是否已達到足夠之清潔度。除了可以測出細菌殘留外,亦可偵測出在處理器具或裝置上所殘留之食物殘渣,避免因未清潔乾淨而造成細菌滋生,危害食用者的健康。
ATP冷光法較傳統培養節省2天時間
PD-20操作簡易,可直接線上偵測,快速得知結果
比對符合HACCP規範的安全表即可得知樣品清潔程度

掌上型微生物快速ATP冷光檢測儀(不含Kit)
特色:
1. 具有專利保護的ATP/AMP循環檢測技術,增加了檢測的敏感度與穩定性。
2. 重量輕、機身小巧且耐用。
3. 可直接將數據轉換成Excel檔案。
4. 可快速檢測環境安全,即時判斷環境的衛生狀況。
5. PD-20N除了可以檢測微生物的總含量之外,還可以檢測食物中殘留的微生物。
6. 搭配LuciPac Pen檢驗套組,具有穩定性好和操作簡單等特點。
7. LuciPac Pen檢驗套組可以在10秒內得到結果,可即時監控現場衛生狀況。
規格:
1.偵測靈敏度:10-15 mol ATP/assay
2.測量時間:10秒鐘
3.資料輸出單位:RLU (Relative Light Unit)
4.儲存容量:可存放2000個數據
5.顯示:LCD顯示
6.與電腦連接介面:USB
7.印表機:熱感式印表機 (選配)
8.電源:2顆1.5 Volt電池
9.尺寸:65 x 175 x 32 (mm)
10.重量:235 g (不含電池)
掌上型ATP冷光儀專用試劑(100支/box)


 
 
 
 
 
 

Apo-BrdU TUNEL Assay kit 組織或細胞凋亡TUNEL分析。DNA Fragmentation assay 染色體斷裂成碎片,在DNA的斷裂口被末端轉移酶作用,加上一個dUTP


【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

細胞凋亡DNA片段分析Apo DNA Fragmentation Assay Kit
螢光波段設定
實驗時間
偵測方式
    樣品型態
K401-60
(Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI
1小時
流氐細胞 儀或螢光顯微鏡
  懸浮細胞、貼附型細胞、福馬林包埋組織
K404-60
Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD
3小時
流氐細胞儀或螢光顯微鏡
  組織切片或固定細胞
K403-50
immunohistochemical staining
3小時
免疫組織 染色
  組織切片或固定細胞
K402-50
Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI
1小時
流氐細胞 儀或螢光顯微鏡
  懸浮細胞、貼附型細胞

BIOVISION提供一系列Apo-BrdU TUNEL DNA Fragmentation kit。TUNEL分析被廣泛的用於觀察細胞計劃性死亡(apoptosis) 之現象。TUNEL的英文全名為Terminal transferase dUTP Nick End Labeling。由Garvrieli, Sherman, 及 Ben-Sasson三位科學家發表於1992發表於J Cell Biol.期刊。

[TUNEL分析產品作用原理]
 
TUNEL之作用原理: 細胞在計劃性死亡(或細胞自然凋亡)時,會使染色體(chromosome)斷裂成碎片狀(fragmentation),並且在DNA的斷裂口(Nick)由terminal transferase(末端轉移酶)作用,加上一個dUTP。
 
[免疫組織專用產品]#K403-50
 


K403-50
50 assays

BIOVISION公司提供的Apo-BrdU IHC kit#k403-50,應用高專一性的抗BrdU抗體,專一性辨視dUTP位置。為了在IHC免疫染色過程中,增加呈色的效果及訊號,BIOVISION在抗BrdU抗體上連結BIOTIN,藉由BIOTIN可與strep-Avidin-HRP結合,增強及改善抗體的訊號,讓TUNEL 分析更容易。所以在顯微鏡下DNA的斷裂處就會呈現褐色,可以辨識細胞是否有自然凋亡的現象。產品附有methyl green solution可計算細胞染色。
產品特點:
1. Apo-BrdU IHC應用高專一性的抗BrdU抗體,專一性辨視dUTP位置。在IHC免疫染色過程中,增加呈色的效果及訊號。
2. 附有positive control 玻片及negative control 玻片,掌控實驗的進行可應用於組織檢體或細胞樣品。




3. 可應用於組織切片或固定後之細胞
[螢光顯微鏡及流氐細胞儀專用TUNEL分析產品]
 
K401-60
     60 assays
K402-50
       50 assays
K404-60
60 assays




[螢光顯微鏡及流氐細胞儀專用TUNEL分析產品]-K401-60
K401-60 ApoBrdU DNA Fragmentation Assay Kit
偵測方式: 流氐細胞儀或螢光顯微鏡
細胞條件: 懸浮細胞、貼附型細胞、福馬林包埋組織
實驗流程: 1小時
螢光波段設定: Ex/Em = 488/520 nm for FITC and 488/623 nm for PI
K401-60產品規格
• Positive Control Cells
• Negative Control Cells
• Wash Buffer
• Reaction Buffer
• TdT Enzymes
• Br-dUTP
• Rinse Buffer
• Anti-BrdU-FITC Antibody
• PI/RNase Staining Buffer

[螢光顯微鏡及流氐細胞儀專用TUNEL分析產品]-K404-60
K404-60 ApoBrdU Red DNA Fragmentation Kit
偵測方式: 流氐細胞儀或螢光顯微鏡
細胞條件: 組織切片或固定後細胞
實驗流程: 3小時
螢光波段設定: Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD
K404-60產品規格
• Positive Control Cells
• Negative Control Cells
• Wash Buffer
• Reaction Buffer
• TdT Enzymes
• Br-dUTP
• Rinse Buffer
• Anti-BrdU-Red Mouse IgG1
• 7-AAD/RNase Staining Buffer

[螢光顯微鏡及流氐細胞儀專用TUNEL分析產品]-K404-60
K402-50 ApoDIRECT DNA Fragmentation Assay Kit
偵測方式: 流氐細胞儀或螢光顯微鏡
細胞條件: 懸浮細胞、貼附型細胞
實驗流程: 1小時
螢光波段設定: Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD
K402-50產品規格
• Positive Control Cells
• Negative Control Cells
• Wash Buffer
• Reaction Buffer
• TdT Enzymes
• FITC-dUTP
• Rinse Buffer
• PI/RNase Staining Buffer



BioVision產品覆蓋細胞增殖,細胞損傷和修復,糖尿病,肥胖和代謝綜合症,幹細胞生物學,基因調控,信號轉導等。代理商萊恩生物科技有限公司





BioVision在開發和研究細胞凋亡,代謝方面具有卓越的影響力。其產品包括檢測試劑盒,抗體,重組蛋白和酵素等多種產品,以及更多的研究分析工具。除了細胞凋亡和代謝分析方面的傳統領域,BioVision的高品質產品還覆蓋細胞增殖,細胞應激,細胞損傷和修復,糖尿病,肥胖和代謝綜合症,幹細胞生物學,基因調控,信號轉導等多個細胞生物學領域。BioVision的產品銷售全球60多個國家。太鼎生物科技公司為BioVision在台灣區域的總代理商,提供最完整的細胞分析試劑盒、重組蛋白及相關抗體產品,以及更快捷和完善的售前售後服務。
BioVision的眾多創新性的試劑盒產品在全球享有盛譽,其Annexin V雙染試劑盒至今仍然全球眾多實驗室被作為凋亡檢測的標杆產品來使用,而種類眾多的Caspase試劑盒則提供了最完整的從Caspase活性檢測、 Caspase蛋白定量到Caspase酶蛋白、Caspase抑制物和底物等多種不同類型的產品,滿足您實驗的需要。包括葡萄糖、果糖、半乳糖到膽固醇、尿素、cAMP等不同類型的代謝分子或代謝中間產物,均有合適的產品提供。

另外,BioVision的胞器蛋白萃取和分離試劑也具有明顯的優勢和特色,包括各種蛋白提取試劑盒,如總蛋白、胞漿蛋白、胞核蛋白、線粒體、膜蛋白、細胞器蛋白提取試劑盒以及線粒體DNA提 取試劑盒。另有細胞信號轉導研究的試劑盒、抗體以及拮抗蛋白等產品,以及細胞因數和生長因數等產品。
BioVision提供的氧化應激和損傷類的產品也被廣泛應用,其產品包括GSH, GST, NAD, NADH, NO,H₂O₂, SOD, Ascorbic, Catalase, Glutamate, Malate, Nitric Oxide等多種氧化損傷分子及指標的檢測。
【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

細胞凋亡產品種類眾多,如何選擇適合的Apoptosis assay kit,Biovsion提供你最快的產品訊息。依樣品種類、觀察細胞的不同位置、研究的目的不同,細分有幾十種產品。


【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com


細胞凋亡分析
Apoptosis, Cell Death, and Cell Proliferation
Many assays exist to measure apoptosis, cell death and cell proliferation. However, if you have only recently become interested in this area, you may find the diversity of assays for measuring apoptosis, cell death, and cell proliferation bewildering. Here we present our Apoptosis Assay Selection Guide to help you choose the best assays for your purposes.
依你的樣品來選擇適合的分析套組
My sample is...
ProductCat#
Live Cells
(Suspension or Adherent)
Cell Lysate
(Fresh or Frozen)
Tissue Lysate
(Fresh or Frozen)
Tissue Sections
(Frozen of paraffin)

I want to measure...
ProductCat#
Mitochondrial Changes
Membrane Changes
Nuclear Changes
Cytosol Changes

My purpose is to...
ProductCat#
Measure Cytochrome c Release
Screen Caspase Inhibitors
Block Apoptotic Gene
Expression
Study Apoptosis Related
Non-Caspase Proteases
Isolate Apoptotic Cells
Induce Apoptosis
Differentiate between Apoptosis & Necrosis

【細胞凋亡抗體】Caspase-8 (Active) Antibody,貨號3259-100 CASP8 , MGC78473 , CASP-8, MACH, procaspase-8,

【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

Caspase-8 (Active) Antibody
Catalog#: 3259-100 | Size: 100 µg


Antibody Target: Caspase-8 (Active) Alternative Name: CASP8 , MGC78473 , CASP-8, MACH, procaspase-8, MCH5, ALPS2B, FLICE, CAP4, EC 3.4.22.61 Host: Rabbit Antibody Type: Polyclonal Isotype: Rabbit IgG Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 392 of mouse caspase-8 Accession#: BC006737 Gene ID: 12370 Appearance: Colorless liquid Concentration: 0.5 mg/ml Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in PBS containing 50% glycerol, 0.5% BSA, and 0.01% thimerosal. Purification: Affinity purified Species Reactivity: mouse, rat Application: Western blot Application & Usage: Western blotting (0.5-4 µg/ml) in samples from mouse and rat origins. However, the optimal conditions should be determined individually. The antibody detects the 25 kDa large subunit of caspase-8. It does not detect the full-length of caspase-8. Storage Temp.: -20 °C Shipping: gel pack Background Descriptions: Caspase-8, a member of the caspase-family of proteases, plays a key role in mediating Fas (CD95) and TNF induced apoptosis. Caspase-8 is synthesized as inactive pro-enzyme and activation of the enzyme involves proteolytic cleavage that leads to the release of the active p18 and p10 subunits. Activated caspase-8 is able to cleave and activate downstream caspases, such as caspase-3, -6, -7 and a death agonist member of the Bcl-2/Bcl-xL family, Bid, leading to apoptosis. Handling: The antibody solution should be gently mixed before use. Usage: For Research Use Only! Not to be used in humans.
Related Products:

Fluorescent proteins, Protein A/G/L & Affinity Conjugates, Secondary Antibodies, Tag Antibodies, Western Blot Substrate Kit, Loading Control 等多種蛋白質相關研究

Fluorescent Proteins

Fluorescent proteins (e.g., EGFP) have become well established marker for gene expression and protein targeting in intact cells and organisms. BioVision offers the brightest Green / Red / Blue / Cyan / Yellow / mCherry fluorescence proteins that expressed from E. Coli. These recombinant proteins were purified using a proprietary technology that ensures highest purity and fluorescence intensity. Fluorescence proteins are very useful in gene expression and many other applications including: Use as standards for SDS-PAGE, Western blot analysis, fluorometer and flow cytometer calibration, fluorescence microscopy, and microinjection into cells and tissues, etc. The recombinant fluorescence proteins can also be conjugated to other proteins. Antibodies against these proteins and mammalian expression vectors, as well as GFP quantification kit area also available.

BFP
CFP
EGFP
RFP (dsRed)
RFP (mCherry)
YFP
Fluorescent Proteins Set
Fluorescent Protein Antibodies Set

 
Protein A/G/L & Affinity Conjugates
BioVision offers unique recombinant protein A, G, L. These genetically engineered proteins contain only the IgG binding regions and have 6X His-tag on their N-terminus. Other domains such as cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from all these three proteins to ensure the maximum specific IgG binding. The 6X His-tag on N-terminus of these proteins can be used for affinity purification or for protein A/G/L detection using anti-His-tag antibody (Cat.# 3998-100). These unique, high quality recombinant Protein A, G and L are produced in E. coli, and purified using multiple chromatographic steps (not affinity purified with human IgG). Purity is > 98% by SDS-PAGE and HPLC. Endotoxin level is < 0.05 EU/mg of Protein. Protein A, G and L bind to most mammalian immunoglobulins in their Fc binding domains and therefore are ideal for purification of polyclonal or monoclonal IgG antibodies. The combinations of Protein A/G and Protein A/G/L are also available, allowing the maximum recovery during antibody purifications.


Protein A
Protein A/G Combination
Protein A/G/L Combination
Protein G
Protein L
Isorbins

 

Related Products:
 
 
【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

BioVision開發各種檢測細胞凋亡(apoptosis, programmed cell death)分析套組,可檢測早,中,後期的細胞凋亡,包括細胞膜,細胞質,線粒體,細胞核不同細胞部位之分析。





BioVision開發各種檢測細胞凋亡(apoptosis, programmed cell death)分析套組,可檢測早,中,後期的細胞凋亡,包括細胞膜,細胞質,線粒體,細胞核不同細胞部位之分析。




細胞凋亡(apoptosis),或程序性細胞死亡(programmed cell death),在許多生理和疾病扮演重要角色。目前監測細胞的細胞凋亡的進展、篩選具有調節細胞凋亡的途徑的新藥物,已是基礎的研究所必須。 BioVision的已開發了各種檢測,可以檢測早,中,後期的細胞凋亡,並檢測凋亡的細胞,在不同的細胞部位,包括細胞膜,細胞質,線粒體,細胞核。

【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com